Figure 7
(a) Orthoslice through a macrophage, reconstructed by filtered back-projection from projections which were phased by single-distance CTF, without any tomographic alignment processing. (b) The same slice as in (a) but with tomographic consistency enforced by alignment algorithms. (c) Combined iterative reprojection phase retrieval (IRP) of the cell, as described in the main text. (d) Three-dimensional rendering of the IRP reconstruction. From Krenkel (2015). Scale bars denote 5 µm. |