Figure 5
Comparison of electron-density maps after density modification in Autosol (Terwilliger et al., 2009) of the CR survivin data using multivariate (σ = 1.0, ρ = 0.26 e Å−3) (a) and univariate (σ = 1.0, ρ = 0.29 e Å−3) (b) analysis. Multivariate analysis of CR data gives the most accurate electron-density map. The figure was generated by the program PyMOL (Schrodinger, 2015) by extending the initial electron-density map of CR multivariate (a) and univariate (b) analysis with fast Fourier transform software (fft) from the CCP4 package (Winn et al., 2011). Both images are equally oriented and visualized in an orthoscopic view. The survivin structural model (black) corresponds to the final refined model obtained from the CR multivariate data set. |