Structure of the nucleotide-binding domain of Plasmodium falciparum Rab6 in the GDP-bound form

The 2.3 Å resolution structure of the nucleotide-binding domain of P. falciparum Rab6 in GDP-bound form was determined using the MAD phasing technique.

Structure of the Fab fragment from F124, a monoclonal antibody specific for hepatitis B surface antigen

The crystal structure of the Fab fragment from the monoclonal anti-preS2 antibody has been solved by molecular replacement and refined at 3.0 Å resolution.

Crystallization, structure solution and refinement of hen egg-white lysozyme at pH 8.0 in the presence of MPD

Tetragonal lysozyme crystals have been grown at pH 8.0 using MPD as a precipitant. The observed difference in growth behaviour relative to the crystal form that grows at pH 4.5 in the presence of NaCl may be a consequence of the presence of a (4R)-MPD molecule bound between two lysozyme molecules in the crystal lattice.

Effects of crystal twinning on the ability to solve a macromolecular structure using multiwavelength anomalous diffraction

The utilization of data collected on pseudo-merohedrally twinned crystals of a small protein for structure solution using multiwavelength anomalous diffraction is discussed and various phasing programs are compared.

Maximum-likelihood density modification

A likelihood-based density modification approach is developed that can incorporate expected electron-density information from a wide variety of sources.

Towards automated Laue data processing: application to the choice of optimal X-ray spectrum

Laue data can now be processed in a nearly automated way. Soft-limited predictions significantly improve the quality of Laue data. A comparative study reveals that single-line undulators are the insertion devices of choice for time-resolved Laue experiments on macromolecules.

The high-mosaicity illusion: revealing the true physical characteristics of macromolecular crystals

Rapid statistical quantitative sampling of crystal mosaicity is accomplished with a CCD detector and unfocused synchrotron source by collecting rotation data in superfine φ increments of 0.001°.

Cryosalts: suppression of ice formation in macromolecular crystallography

The use of lithium formate, lithium chloride and other highly soluble salts in suppression of the formation of crystalline or microcrystalline ice in macromolecular cryocrystallography is described.

Implementation of cluster analysis for ab initio phasing using the molecular envelope from solution X-ray scattering

The low-resolution molecular shape of superoxide dismutase determined from solution X-ray scattering data was located in the crystallographic unit cell by a molecular-search method. The absent reflections were replaced by randomly generated intensity values and a cluster analysis was used to select the solution.

Crystallization and molecular-replacement studies of a recombinant antigen-binding fragment complexed with single-stranded DNA

Crystals of an antigen-binding fragment complexed with dT₅ have been obtained and a molecular-replacement solution has been determined. AMoRe performed better than X-PLOR when using small search models.

Crystallization and preliminary X-ray analyses of insect neurotoxins with analgesic effect from the scorpion Buthus martensii Karsch

Three insect neurotoxins with analgesic effect from the scorpion B. martensii Karsch have been purified and crystallized in hexagonal, tetragonal, monoclinic and rhombohedral forms. Preliminary X-ray data are presented.

Crystallization and diffraction to ultrahigh resolution (0.8 Å) of a designed variant of the Rop protein

Crystals of a designed Rop variant have been obtained and X-ray data have been collected to a resolution of 0.8 Å.

Plasmodium falciparum Rab6 GTPase: expression, purification, crystallization and preliminary crystallographic studies

Rab6 GTPase from the organism responsible for causing malaria, P. falciparum, has been expressed, purified and crystallized in two different forms. Preliminary crystallographic data have also been obtained.

Crystallization and X-ray diffraction analysis of peroxisomal Δ³-Δ²-enoyl-CoA isomerase from Saccharomyces cerevisiae

S. cerevisiae Δ³-Δ²-enoyl-CoA isomerase has been crystallized and a native data set has been collected at 2.5 Å.

Crystallization and preliminary X-ray analysis of Clostridium botulinum neurotoxin type B

C. botulinum neurotoxin B has been crystallized and native data have been collected to 1.8 Å resolution.

Crystallization and preliminary X-ray analysis of the Clostridium thermocellum cellulosome xylanase Z feruloyl esterase domain

Crystals of C. thermocellum xylanase Z feruloyl esterase have been obtained that diffract to 2.4 Å resolution. The lattice is ortho­rhombic, space group P2₁2₁2₁, with unit-cell parameters a = 43.14, b = 63.77, c = 79.57 Å.

Crystallization and preliminary X-ray diffraction studies of the signal recognition particle receptor FtsY from Mycoplasma mycoides

One of the proteins from the prokaryotic signal recognition pathway, FtsY, has been studied and results are presented here. Four versions of the FtsY receptor protein from M. mycoides have been cloned, expressed in E. coli and purified. Purified proteins were then subjected to crystallization trials and preliminary data obtained.

Crystallization and preliminary X-ray analysis of the conserved domain IV of Escherichia coli 4.5S RNA

Crystals are described of the conserved domain IV of E. coli 4.5S RNA, which together with Ffh protein constitutes the bacterial signal recognition particle (SRP) and, by independently binding to elongation factor G (EF-G), also plays an essential role in ribosomal translocation.

A crystallizable form of RIIβ regulatory domain obtained by limited proteolysis

A crystallizable form of the RIIβ regulatory subunit of PKA was obtained by limited proteolysis of the full-length subunit. Ortho­rhombic crystals were grown which diffracted to 2.3 Å.

Crystallization and preliminary crystallographic analysis of the Rho-binding domain of bovine Rho-kinase

Crystals of the Rho-binding domain of Rho-kinase were obtained in both native and selenomethionyl forms. The crystals belong to the space group C2 [a = 148.0 (2), b = 26.1 (1), c = 39.6 (1) Å, β = 90.3 (1)°] and diffract to a resolution beyond 1.5 Å.

Crystallization and preliminary X-ray crystallographic analysis of PdxJ, the pyridoxine 5′-phosphate synthesizing enzyme

The enzyme PdxJ, which is involved in synthesis of pyridoxine, has been crystallized in several forms under different conditions. The best crystals have undergone crystallographic analysis.

Crystallization and preliminary X-ray crystallographic studies on maltosyltransferase from Thermotoga maritima

Recombinant maltosyltransferase from the hyperthermophilic bacterium T. maritima has been crystallized. The crystals belong to space group P4₁22 or P4₃22 and diffract to at least 2.4 Å resolution.

Crystallization and preliminary crystallographic investigations of avian 5-amino­imidazole-4-carboxamide ribonucleotide transformylase–inosine monophosphate cyclo­hydrolase expressed in Escherichia coli

Crystals of the de novo purine biosynthesis enzyme ATIC in complex with methotrexate have been obtained. X-ray data have been collected to 2.3 Å at Stanford Synchrotron Radiation Laboratory.

Preliminary crystallographic study of turkey gizzard vinculin

Vinculin, an important protein in the maintenance of the actin cytoskeleton and cell–cell adhesion, has been isolated from smooth muscle, and crystals have been obtained.

Purification, crystallization and preliminary X-ray study of β-xylosidase from Trichoderma reesei

An extracellular β-xylosidase from T. reesei has been purified and crystallized. The crystal belonged to an orthorhombic space group and diffracted to 2.7 Å resolution.

Crystallization and structure determination of the catalytic trimer of Methanococcus jannaschii aspartate transcarbamoylase

The catalytic trimer of M. jannaschii aspartate transcarbamoylase has been crystallized. The work leading to the determination of its structure by molecular-replacement and Patterson methods is described.

Apocrustacyanin A1 from the lobster carotenoprotein α-crustacyanin: crystallization and initial X-ray analysis involving softer X-rays

Apocrustacyanin A1 is a 20 kDa subunit of the blue lobster carapace carotenoprotein α–crustacyanin, which provides the animal with cryptic colouration, is a member of the lipocalin superfamily and whose bathochromic spectral shift of the bound carotenoid is of similar magnitude to that for retinal in the visual pigments. Data recorded using softer X-rays (2.0 Å wavelength) are also reported as a strategy to tackle the challenge of 40 kDa in the asymmetric unit.

A neutralizing antibody Fab–influenza haemagglutinin complex with an unprecedented 2:1 stoichiometry: characterization and crystallization

A complex of the influenza virus haemagglutinin trimer with the Fab of a neutralizing antibody has been shown to have a 1:2 stoichiometry. Diffraction data were collected to 3.5 Å resolution and the initial structure was solved by molecular replacement.

On the distribution of the bulk-solvent correction parameters

Analysis of the bulk-solvent correction parameters (B_sol, k_sol) obtained from 219 crystal structures deposited with the PDB shows that current refinement methods fail to uncouple the two parameters, resulting in values that have no physical meaning.

Molecular-replacement studies of Trichosanthes kirilowii lectin 1: a structure belonging to the family of type 2 ribosome-inactivating proteins

Molecular-replacement studies of T. kirilowii lectin 1 show that its structure belongs to the family of type 2 ribosome-inactivating proteins.

Purification, crystallization and preliminary crystallographic studies of an integral membrane protein, cytochrome bo₃ ubiquinol oxidase from Escherichia coli

The integral membrane protein cytochrome bo₃ ubiquinol oxidase was crystallized in space group C222₁, with cell dimensions a = 91.3, b = 370.3 and c = 232.4 Å. A clear molecular-replacement solution was obtained.

Enhancement in the perfection of orthorhombic lysozyme crystals grown in a high magnetic field (10 T)

Orthorhombic lysozyme crystals grown in a high magnetic field showed improvements of both higher resolution and lower mosaicity for X-ray analysis.

Crystallography made crystal clear (2nd edition)

Crystallization of nucleic acids and proteins: a practical approach (2nd edition)