Structural effects of monovalent anions on polymorphic lysozyme crystals

Anion sites in lysozyme polymorphic structures were classified as sequence-dependent, packing-dependent and unique non-specific sites.

Intermonomer interactions in dimer of bovine heart cytochrome c oxidase

Nuclear-coded subunits stabilize a stable dimeric state in bovine heart cytochrome c oxidase.

Structure of aspergillopepsin I from Aspergillus phoenicis: variations of the S1′–S2 subsite in aspartic proteinases

The crystal structure of aspergillopepsin I from A. phoenicis has been determined at 2.18 Å resolution. In the combined S1′–S2 subsite, aspergillopepsin I is similar to endothiapepsin but differs from penicillopepsin, which shares the higher sequence identity.

Structure of a new polymorphic monoclinic form of human transthyretin at 3 Å resolution reveals a mixed complex between unliganded and T₄-bound tetramers of TTR

A new polymorphic form of human transthyretin contains a unique assembly of apo TTR and TTR–T₄ complex in the asymmetric unit of the monoclinic lattice. These data provide insight into the mechanism of negative cooperativity in T₄ binding.

Structure of ribosomal protein TL5 complexed with RNA provides new insights into the CTC family of stress proteins

The crystal structure of ribosomal protein TL5 is the first structure of a full-size protein from the CTC family of stress proteins. Structure of TL5 complexed with RNA reveals new features of protein–RNA binding.

De novo structure determination of vancomycin aglycon using the anomalous scattering of chlorine

The anomalous scattering of chlorine has been exploited to determine the structure of the macromolecular antibiotic vancomycin aglycon.

High-resolution neutron structure of nicotinamide adenine dinucleotide

The crystal structure of NAD⁺ was determined by a neutron-diffraction experiment. The stereochemistry, hydrogen bonds and C—H⋯O interactions are described and compared with several high-resolution NAD⁺ holoenzyme structures.

Anomalous signal of phosphorus used for phasing DNA oligomer: importance of data redundancy

The crystal structure of a left-handed Z-DNA duplex has been solved based on the anomalous signal of the P atoms with single-wavelength data collected at 1.54 Å. The redundancy of measurements crucially affects the phasing process.

Protein crystal structure solution by fast incorporation of negatively and positively charged anomalous scatterers

The use of two different classes of compounds (monovalent and polyvalent cations) in the quick cryosoaking procedure for derivatization and phasing of protein crystals has been proposed. This approach was tested on hen egg-white lysozyme and was successfully employed to solve the structure of a novel protein.

The absorption edge of protein-bound mercury and a double-edge strategy for HgMAD data acquisition

A double-edge approach with a set of four wavelengths [1.0087 Å (12 291 eV, edge1), 1.0084 Å (12 295 eV, edge2), 1.0064 Å (12 320 eV, peak) and 0.9918 Å (12 500 eV, remote)] is recommended for HgMAD data acquisition.

Using surface-bound rubidium ions for protein phasing

The anomalous signal from rubidium ions bound to the protein surface was used for phasing of the crystal structure of the hsp60 apical domain from T. thermophilus. Phase quality was sufficient for autotracing of the protein model at 2.0 Å resolution.

A new software routine that automates the fitting of protein X-ray crystallographic electron-density maps

This routine automates the steps that are usually followed by an investigator working at a graphics terminal. It is applicable to relatively low resolution maps (2.5 to 2.8 Å).

Effects of PEG on detergent micelles: implications for the crystallization of integral membrane proteins

PEG is shown to promote micelle–micelle attraction and increase apparent micelle size, suggesting two mechanisms by which detergent effects can modulate the crystallization of integral membrane proteins.

Preliminary crystallographic study of Thermus aquaticus glycerol kinase

Crystals of T. aquaticus GlpK have been obtained by the hanging-drop method. The crystals diffract to 2.87 Å resolution and belong to space group I23.

Crystallization and preliminary X-ray analysis of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus

Diffraction-quality crystals of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from S. aureus have been prepared. The crystals belong to the cubic space group I2₁3, with unit-cell parameters a = b = c = 178.99 Å.

Crystallization and X-ray diffraction measurements of a thermophilic archaeal recombinant amidase from Sulfolobus solfataricus MT4

A recombinant thermophilic archaeal amidase from S. solfataricus MT4 that catalyzes the hydrolysis of aliphatic and aromatic amides has been crystallized. Diffraction data for the native protein (to 2.55 Å) and a putative derivative (to 2.20 Å) have been collected.

Cloning, purification, crystallization and preliminary X-ray diffraction analysis of the antistasin-type inhibitor ghilanten (domain I) from Haementeria ghilianii in complex with porcine β-trypsin

The synthetic gene of the amino-terminal domain of the antistasin-type inhibitor ghilanten (ghilanten-D1) from H. ghilianii was constructed, expressed in the methylotrophic yeast P. pastoris and purified by heparin–Sepharose chromatography. Recombinant ghilanten-D1 has been crystallized in complex with porcine β-trypsin in three different-looking but isomorphous crystal forms, each belonging to the orthorhombic space group P2₁2₁2₁.

Characterization and crystallization of a novel Sarcocystis muris lectin, SML-2

A novel lectin SML-2 consisting of 138 amino acids was isolated from cyst merozoites of S. muris and sequenced by Edman degradation and mass spectrometry. Crystals diffracting to 2.1 Å resolution at a synchrotron were grown by the hanging-drop vapour-diffusion technique.

Crystallization and preliminary X-ray crystallographic studies on 4-α-glucanotransferase from Thermotoga maritima

4-α-Glucanotransferase from the hyperthermophilic bacterium T. maritima has been crystallized. The crystals belong to an I-centered orthorhombic space group and diffract to at least 2.6 Å resolution.

An improved phase-extension procedure for isomorphous replacement phases

A new phase-extension with density modification procedure to improve isomorphous replacement phases is described.

Oxford handbook of nucleic acid structure