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Figure 1
(a) Nucleic acid scaffold used to assemble T7 RNA polymerase elongation complex. (b) PAGE analysis of the crystals. Samples were resolved in 4–12% gradient gels (Nu-PAGE, Invitrogen) using an MES buffer system and visualized by silver staining. Lane 1, Mark-12 molecular-weight standards (Invitrogen); lane 2, RNA–DNA scaffold; lane 3, crystal; lane 4, T7 RNA polymerase.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
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