 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](bw5043fig1mag.jpg)
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Figure 1
Purification of the complex of H-2Kd with HBV core 87–96 by FPLC Superdex G75 gel-filtration and Mono Q anion-exchange chromatography. (a) Refolding attempt without peptide. The first peak represents aggregated heavy chain (labelled 1) and the second peak β2m (labelled 3). (b) Refolding in the presence of peptide (SYVNTNMGL). In comparison with the profile in (a), peak 2 represents the correctly refolded H-2Kd complex (45 kDa). (c) Further purification of the refolded complex by anion exchange. Peak 4 represents the H-2Kd complex, which was eluted at a NaCl concentration of 17–26 mM. (d) SDS–PAGE gel (15%) of the purified complex. Lane 1, H-2Kd inclusion bodies; lane 2, β2m inclusion bodies; lane 3, protein standard markers in kDa; lane 4, the purified refolded H-2Kd complex, showing bands for H-2Kd and β2m. |
![[Figure 1]](bw5043fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
