|
Figure 1
Crystals obtained under various conditions reported in supplementary material Table A . Row 1, left to right: conditions 4 [C-KARI with 5%(w/v) PEG 6000, 0.1 M sodium citrate pH 5.0], 6 [C-KARI with 10%(v/v) isopropanol, 10%(w/v) PEG 4000, 0.1 M sodium citrate pH 5.6] and 19 [C-KARI with 10%(w/v) PEG 3000, 0.2 M MgCl2, 0.1 M sodium cacodylate pH 6.5]; row 2, 16 [C-KARI with 20%(w/v) PEG 6000, 0.1 M Na Bicine pH 9.0], 20 [C-KARI with 20%(w/v) PEG 1000, 0.2 M MgCl2, 0.1 M sodium cacodylate pH 6.5] and 54 [SeMet-N-KARI with 15%(w/v) PEG 4000, 0.2 M Li2SO4, 0.1 M Tris–HCl pH 8.5]; row 3, 22 [C-KARI with NADPH, 10%(w/v) PEG 8000, 0.2 M NaCl, 0.1 M sodium/potassium phosphate pH 6.2], 24 [C-KARI with NADPH, 10%(w/v) PEG 3000, 0.2 M MgCl2, 0.1 M sodium cacodylate pH 6.5] and 35 [N-KARI with NADPH, 30%(v/v) PEG MME 550, 0.1 M NaCl, 0.1 M Na Bicine pH 9.0]; row 4, 23 [C-KARI with NADPH, 1.6 M (NH4)2SO4, 0.1 M Na Bicine pH 9.0], 26 [N-KARI with 10%(w/v) PEG 6000, 4.8%(v/v) MPD, 0.1 M Na HEPES pH 7.5] and 33 [N-KARI with NADPH, 0.68 M trisodium citrate, 0.1 M Tris–HCl pH 8.5]; row 5, 27 [N-KARI with 15%(w/v) PEG 4000, 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.5], 45 [SeMet-N-KARI with 20%(w/v) PEG 3000, 0.1 M sodium citrate pH 5.5] and 55 [SeMet-N-KARI with 15%(w/v) PEG 4000, 0.2 M sodium acetate, 0.1 M Tris–HCl pH 8.5]. |