Three-dimensional structure determination of proteins related to human health in their functional context at The Israel Structural Proteomics Center (ISPC)

Albeck, S.; Burstein, Y.; Dym, O.; Jacobovitch, Y.; Levi, N.; Meged, R.; Michael, Y.; Peleg, Y.; Prilusky, J.; Schreiber, G.; Silman, I.; Unger, T.; Sussman, J.L.

doi:10.1107/S0907444905023565

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 6
Temperature optimization in E. coli expression of two targets, W00003 and W00006, cloned in three different vectors, using His, Trx and GST tags, at two temperatures. Expression at 288 K resulted in a higher proportion of soluble protein (red asterisk). P, pellet; S, soluble.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 61| Part 10| September 2005| Pages 1364-1372

doi:10.1107/S0907444905023565

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