Three-dimensional structure determination of proteins related to human health in their functional context at The Israel Structural Proteomics Center (ISPC)

Albeck, S.; Burstein, Y.; Dym, O.; Jacobovitch, Y.; Levi, N.; Meged, R.; Michael, Y.; Peleg, Y.; Prilusky, J.; Schreiber, G.; Silman, I.; Unger, T.; Sussman, J.L.

doi:10.1107/S0907444905023565

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 7
Optimization of the induction medium for protein expression in P. pastoris. Target W00075 was expressed in P. pastoris in three media, BMMY, BMM and MM, induction taking place with methanol at 293 K. Samples were taken at three different times points during induction, 72, 120 and 144 h (from left to right in each medium), and were analyzed by SDS–PAGE, stained with Gel Code (left) or subjected to Western analysis with anti-His₆ monoclonal antibodies (right). It can be seen that the choice of medium strongly influences both the amount and quality of the protein produced.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 61| Part 10| September 2005| Pages 1364-1372

doi:10.1107/S0907444905023565

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