 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 3]](be5044fig3mag.jpg)
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Figure 3
The structure of the Dsk2 UBA domain. (a) Details of the interactions at the Dsk2 UBA–UBA interface. Secondary-structural elements are labelled: α0, V327–R332; α1, E333–M342; α2, F347–R356; α3, G361–L369. See text for further details. (b) The association of two UBA molecules involves electrostatic interactions. van der Waals surface representation of two UBA molecules with electrostatic potential superimposed (figure produced with electrosurface routine in AESOP; J. Gruber & M. E. M. Noble, unpublished program). The view is similar to that of Fig. 3 ![[link]](../../../../../../logos/arrows/d_arr.gif) (a). The left molecule has a transparent surface to show the UBA secondary structure and side chains are in green. The Dsk2 UBA molecule has an asymmetric charge distribution with a cluster of negatively charged residues to the left (E329, E330, E333, D346 and D348) and a cluster of positively charged residues to the right (R331, R355 and R356). The complementary charged surfaces interact in the crystal to form helical polymers. (c) Structure-based sequence comparison for UBA domains from S. cerevisiae Dsk2, human PLIC2, Schizosaccharomyces pombe Mud1, human HR23A UBA domains 1 and 2 and S. cerevisiae Cue2. Secondary-structure elements for Dsk2 UBA are indicated. The negatively and positively charged residues that form the interacting surfaces for Dsk2 UBA polymers are indicated in red and blue, respectively. These residues are not conserved in other UBA domains. Residues that contact UBL in the UBA–UBL complex are highlighted in cyan. |
![[Figure 3]](be5044fig3.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
