issue contents

Journal logoBIOLOGICAL
ISSN: 1399-0047

February 2006 issue

Highlighted illustration

Cover illustration: Selective fluorescent staining of nucleic acid-containing crystals. A comparison of core Pol II protein crystals in the free form (P) and in complex with an 80-mer RNA inhibitor (C) is shown. On the right, a transmission light microscopic image reveals all crystals. On the left, a fluorescence image shows selective staining of nucleic acid complex crystals after incubation with SYBR-Gold (p. 146).

research papers

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The crystal structure of human dCK in complex with clofarabine reveals the structural basis of activation by purine-nucleoside prodrugs.

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The crystal structure of T. gondii adenosine kinase in complex with an ATP analog at 1.1 Å resolution reveals that ATP binding and the formation of the induced anion hole can occur before adenosine binding.

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A highly sensitive and specific fluorescence-based assay for the rapid detection of nucleic acids in crystals of macromolecular complexes is described.

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The structure of the manganese form of prostaglandin H2 synthase-1 reveals that metal substitution has no effect on the enzyme's structure; however, the manganese is displaced farther out of the porphyrin plane than is the native iron.

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The structure of the uropathogenic E. coli invasin DraD containing a C-terminal extension of 13 amino acids has been solved at 1.05 Å resolution. It forms dimers through the exchange of the C-terminal donor strands, resulting in a variant of the immunoglobulin fold.

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The X-ray crystal structure of the previously unknown bacteriophage P22 lysozyme was determined ab initio by direct methods using the program SIR2002. The asymmetric unit contained 2268 non-H protein atoms, making this one of the largest structures determined to date by ab initio direct methods.

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The structures of Dsk2 UBL, Dsk2 UBA and their complex are described. The complex explains the reduced affinity of the UBA domain for UBL compared with that for ubiquitin.

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A manual intervention-free automatic refinement software package (LAFIRE) has been developed for high-throughput protein-structure analysis. This program begins from an initial model that can be approximate, fragmentary or even only main-chain and performs a whole refinement process to provide a final model including water molecules.

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New crystal structures of glutathione transferase A1-1 show that the C-terminal region of the apo form can be ordered and helix-like. They also show how glutathione is bound in the absence of a second substrate and provide insight into the binding of a decarboxylated glutathione conjugate.

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The highly pseudosymmetric crystal structure of HTLV-1 protease could be solved using low-sequence-identity models with Phaser but, with a single exception, not with standard application of other molecular-replacement programs.

short communications

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The high-resolution structure of thioredoxin from the parasite T. vaginalis has been determined. The redox-active disulfide has been partially photoreduced by the intense synchrotron radiation used in the analysis. Comparisons with human thioredoxin are presented.

international union of crystallography

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