 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 2]](hv5073fig2mag.jpg)
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Figure 2
ITC analysis of 2′P-ADP-ribose binding to KPR at different pH values. ITC experiments are shown for His6-KPR titrated with 2′P-ADP-ribose at 300 K in (a) 0.1 M HEPES–HCl pH 7.7 and (b) 90 mM sodium acetate, 5 mM HEPES pH 4.5. Top panels: raw data for titrations of 1–2 mM ligand into 30–60 µM protein. Each peak corresponds to one injection. An initial 1 µl injection was followed by 35 × 8 µl (a) or 28 × 10 µl injections (b). Bottom panel: integration of the data, corrected for the heat of dilution. The line represents the least-squares fit to the single-site binding model by the Origin program. |
![[Figure 2]](hv5073fig2.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
