Figure 6
The effect of the addition of lipids on LCP crystallization of RCs. (a) The number of conditions producing crystals out of a total of 48 from the ammonium sulfate and Jeffamine grid screen, as a function of increasing amounts of extracted R. sphaeroides lipids (squares, dotted line), polar E. coli lipids (triangles, unbroken line) and polar brain lipid (circles, dashed line). (b) The quality of the RC crystals produced in LCP trials as a function of increasing amounts of lipid additives. Data for extracted R. sphaeroides, polar E. coli and polar brain lipids were averaged. Values plotted represent either the total number of crystals observed (diamonds, unbroken line) or a high-quality (triangles, dotted line), medium-quality (circles, dotted line) or low-quality (squares, dotted line) visual score based on the size, degree of symmetry and edge definition of the crystals obtained. |