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Figure 4
The primary and secondary structure of SmLip and sequence alignment with two homologues. S. marcescens Lip is aligned with the homologous proteins from enteroaggregative E. coli (GenBank CBG37366.1) and P. aeruginosa (NCBI Reference Sequence NP_248770.1, PA0080). The secondary structure of SmLip is depicted with blue arrows for β-strands and red and green cylinders for α-­helices and 310-helices, respectively. Residues conserved in all three sequences are shown in black boxes and those conserved in only two sequences are shown in grey boxes. The start and finish of the lipobox motif are marked by red boxes; the residues at the start and end of the sequence used in the structure analysis (Ala30–Asp176) are shown in blue boxes. The alignment was generated using T-­Coffee (Di Tommaso et al., 2011BB12) in the M-Coffee mode and the figure was prepared using ALINE (Bond & Schüttelkopf, 2009BB5).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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