Figure 3
Rigid-body fitting of a RecA monomer (PDB entry 2rec, brown; Yu & Egelman, 1997) to a simulated 15 Å resolution map of the hexamer (gray) using point-cloud matching. (a) Before and (b) after matching with the Situs matchpt tool. The coarse-grained anchor points (feature vectors, cyan/blue) were generated using vector quantization (Wriggers et al., 1998) in matchpt. The low-resolution map was generated with pdb2vol using a Gaussian kernel convolution. The images were rendered with VMD (Humphrey et al., 1996). |