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![[Figure 1]](ba5179fig1mag.jpg)
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Figure 1
Primary-sequence alignment between 1vgy (chain A) and Cgl1109. The alignment obtained by PROMALS3D (Pei et al., 2008  ) is shown. The first line in each block shows conservation indices for positions with a conservation index above 4. The last two lines show consensus amino-acid sequence (Consensus_aa) and consensus predicted secondary structure (Consensus_ss). The representative sequences are named in magenta and are colored according to predicted secondary structure (red, α-helix; blue, β-strand). The first and last residue numbers of each sequence in each alignment block are shown before and after the sequences, respectively. Consensus-predicted secondary-structure symbols: α-helix, h; β-strand, e. Consensus amino-acid symbols are as follows (conserved amino acids are shown in bold uppercase letters); aliphatic (I, V, L), l; aromatic (Y, H, W, F), @; hydrophobic (W, F, Y, M, L, I, V, A, C, T, H), h; alcohol (S, T), o; polar residues (D, E, H, K, N, Q, R, S, T), p; tiny (A, G, C, S), t; small (A, G, C, S, V, N, D, T, P), s; bulky residues (E, F, I, K, L, M, Q, R, W, Y), b; positively charged (K, R, H), +; negatively charged (D, E), −; charged (D, E, K, R, H), c. Note that the sequence numbers refer to the genomic sequence of Cgl1109 (taking into account the minor mutations in the construct used for crystallization; see text) and 1vgy. The residue numbering in the deposited PDB file (PDB entry 3tx8) begins with the first residue of the expression construct used, so it is offset by 11 residues compared with the genomic sequence. |
![[Figure 1]](ba5179fig1.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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