Application of DEN refinement and automated model building to a difficult case of molecular-replacement phasing: the structure of a putative succinyl-diaminopimelate desuccinylase from Corynebacterium glutamicum

Brunger, A.T.; Das, D.; Deacon, A.M.; Grant, J.; Terwilliger, T.C.; Read, R.J.; Adams, P.D.; Levitt, M.; Schröder, G.F.

doi:10.1107/S090744491104978X

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 6
Comparison of various refinements and maps for residues 251–276. A close-up view of the loop consisting of residues 264–271, which is also part of Fig. 5

, is shown. The final model is colored orange (sticks and cartoon representation). The structure after the first round of DEN refinement and AutoBuild is colored magenta (sticks and cartoon representation) and the corresponding 2mF_o − DF_c electron-density map (with model phases calculated from this structure, but without experimental phase information, and contoured at 1.4σ) is colored marine blue. The electron-density map clearly shows that the loop needed to be corrected.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 68| Part 4| April 2012| Pages 391-403

https://doi.org/10.1107/S090744491104978X

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