view article
Figure 7
Visual effects of map sharpening on electron density. This example was taken from PDB entry 2r6c. Images show the map with (a) no map sharpening, (b) map sharpening using the inverse filter (no regularization) and (c) a regularized sharpened map using the L2-type Tikhonov regularizer, with sharpening coefficients integrated over B and α, as described in the text. The backbone trace of 2r6c chain C is shown in green. The homologous structure 2r6a chain A is shown in blue, superposed using residues 270–287 from 2r6a chain A. The image shows unmodelled density in 2r6c that corresponds to a helix present in 2r6a. Both sharpened maps show more features than the unsharpened map, with the regularized map giving more connectivity. Images were generated using CCP4mg (McNicholas et al., 2011BB21).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 68| Part 4| April 2012| Pages 404-417
https://doi.org/10.1107/S090744491105606X
Follow Acta Cryst. D
Sign up for e-alerts
E-alerts
Follow Acta Cryst. on Twitter
Twitter
Follow us on facebook
Facebook
Sign up for RSS feeds
RSS