Figure 1
Example of glycosylation, which only requires the generic links for sugars defined in REFMAC's standard monomer library for refinement. The figure shows one molecule of β-L-fucose (FUL) and two molecules of N-acetyl-D-glucosamine (NAG) forming a polysaccharide covalently bound to Asn241 in the crystal structure of the G117H mutant of human butyrylcholinesterase (Nachon et al., 2011; PDB entry 2xmb). Refinement of such a structure will implicitly use the links NAG-ASN (1), BETA1-4 (2), ALPHA1-6 (3) and associated modification from the restraint library. This figure and Fig. 3 were generated using CCP4mg (Potterton et al., 2004). |