The structures of cytosolic and plastid-located glutamine synthetases from Medicago truncatula reveal a common and dynamic architecture

Torreira, E.; Seabra, A.R.; Marriott, H.; Zhou, M.; Llorca, Ó.; Robinson, C.V.; Carvalho, H.G.; Fernández-Tornero, C.; Pereira, P.J.B.

doi:10.1107/S1399004713034718

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 5
Electron cryomicroscopy reconstruction of M. truncatula GSII-2a. (a) FSC showing a resolution of 20 Å. (b) Pairs of class averages (upper row) and projections of the final three-dimensional reconstruction (bottom row). (c) Structure of M. truncatula GSII-2a, with approximate dimensions and positions of the symmetry axes (arrowed lines and pentagon for twofold and fivefold axes, respectively) defining the D5 symmetry. The numbers 1–5 and the numbers in parentheses indicate the positions of the five high-density blobs in the top and bottom rings, respectively. A scheme shows the relative rotation of the pentagons defined by the rings. (d) Fitting of the crystal structure of M. truncatula GSII-1a reported here, in which each monomer is coloured differently.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 70| Part 4| April 2014| Pages 981-993

https://doi.org/10.1107/S1399004713034718

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