Figure 4
Thermograms and binding isotherms with theoretical fits for the binding of (GlcNAc)3–6 to the OfChtI-CAD E148Q mutant: (a) (GlcNAc)3, (b) (GlcNAc)4, (c) (GlcNAc)5, (d) (GlcNAc)6. (e) Free-energy changes for (GlcNAc)3–6 binding to OfChtI-CAD E148Q and (GlcNAc)4–6 binding to SmChiB E144Q (from Norberg et al., 2010) relative to the number of GlcNAc units in the saccharide chain. (GlcNAc)3 binds to OfChtI-CAD E148Q at two sites. S1 is the strong binding site with a low Kd value and S2 is the weak binding site with a high Kd value. |