view article

Figure 2
Overexpression, purification and thermostability of isolated N- and C-terminal globular domains of the Zaire EBOV NP. (a, b) SDS–PAGE gels showing single-step purification of the His-MBP fusion protein (lane 1), the sample following digestion with rTEV (lane 2) and the final sample after removal of the tag and additional purification as specified in §[link]2 (lane 3). (c, d) Results of the thermal stability assays for the NPNt and NPCt domains, respectively. The minimum of the negative derivative of the reporter fluorescence indicates the midpoint of the melting (denaturation) temperature of the protein sample.

Journal logoBIOLOGICAL
ISSN: 1399-0047
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds