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Figure 7
Target arginine-binding pocket in each PRMT type. The active site of MmPRMT7 may accommodate sDMA. Target arginine-binding pockets of (a) MmCARM1 (PDB entry 3b3f ; Troffer-Charlier et al., 2007BB66), (b) HsPRMT5 (PDB entry 4gqb ; Antonysamy et al., 2012BB4) and (c) MmPRMT7. Conserved residues surrounding the pockets are drawn as stick models and labelled. An M48F mutation in type I PRMT1 (corresponding to M163F in MmCARM1) produces sDMA and aDMA. In type II PRMT5, Phe327 is crucial for producing sDMA. A Phe-to-Met mutation produces both sDMA and aDMA. In type I MmCARM1, Met269 may be also important in producing mainly aDMA. This methionine is replaced by a serine (Ser446) in PRMT5. MmPRMT7 shows features similar to type I PRMT and Ala155 may produce space to accommodate aDMA.

Journal logoBIOLOGICAL
CRYSTALLOGRAPHY
ISSN: 1399-0047
Volume 70| Part 9| August 2014| Pages 2401-2412
doi:10.1107/S1399004714014278
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