 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 6]](qh5007fig6mag.jpg)
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Figure 6
Relative selectivity of PvuRts1I and enzyme variants on unmodified cytosine (C), 5-mC and 5-hmC (see § ![[link]](../../../../../../logos/arrows/d_arr.gif) 2 for a description of the methods used). In each gel, the amount of enzyme is titrated from left (high) to right (low). All DNA substrates were made by PCR. (a) PvuRts1I; the approximate relative selectivity is 5-hmC:5-mC:C = 32:4:1. (b) Y210F mutant; the approximate relative selectivity is 5-hmC:5-mC:C = 256:8:1. (c) N217D mutant; the approximate relative selectivity is 5-hmC:5-mC:C = 32:1:1. (d) Comparison of the relative selectivity towards 5-hmC, 5-mC and C for PvuRts1I and the Y210F and N217D mutants. The relative selectivity is plotted on a log scale and normalized based on the activity of C. |
![[Figure 6]](qh5007fig6.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
