Structure of the 2,4′-dihydroxyacetophenone dioxygenase from Alcaligenes sp. 4HAP

Keegan, R.; Lebedev, A.; Erskine, P.; Guo, J.; Wood, S.P.; Hopper, D.J.; Rigby, S.E.J.; Cooper, J.B.

doi:10.1107/S1399004714015053

Acta Crystallographica Section D
Acta Crystallographica
Section D BIOLOGICAL CRYSTALLOGRAPHY

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Figure 6
X-band EPR spectra of DAD. (a) 4000 G field sweep (an asterisk indicates trace Cu²⁺ contamination). (b) A sweep showing the region from 500 to 2100 G, with g values for major lines indicated. (c) DAD with a fivefold molar excess of substrate 2,4′-DHAP added, with g values for major lines indicated; vertical and horizontal scales are as in (b). Experimental parameters: microwave power 0.5 mW, modulation amplitude 5 G, modulation frequency 100 kHz, temperature 10 K; (b) and (c) are the result of four co-added scans.

BIOLOGICAL
CRYSTALLOGRAPHY

ISSN: 1399-0047

Volume 70| Part 9| August 2014| Pages 2444-2454

doi:10.1107/S1399004714015053

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