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Figure 3
Purification of heterododecamers. The peaks are represented using the absorbance (A280 nm) as a function of the conductivity (mS cm−1). (a) Ion-exchange chromatography (Resource Q) of the total sample after the re-oligomerization process. The shaded areas correspond to the fractions grouped together for successive Mono Q purification. (b) Ion-exchange chromatography (Mono Q) of the two major peaks after the first Resource Q step. The shaded areas correspond to the fractions grouped together for size-exclusion chromatography on a Superose 6 column (Supplementary Fig. S10). |
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