Figure 2
Thaumatin screening. Thaumatin crystals grown using a trypsin-MIP. These crystals were obtained using a screen condition that did not yield crystals in control drops [0.2 M ammonium sulfate, 30%(w/v) PEG 8000]. The presence of MIPs facilitated thaumatin crystal formation in a further 14 screen conditions where controls did not form, as detailed in Table 1. 14 of the 15 conditions did not contain tartrate, which is the most potent precipitant for thaumatin crystallization. Visualization of the modified MIPs is challenging. This is primarily owing to their altered consistency with respect to the original MIPs and also owing to the minute volume being added. Furthermore, if any precipitate forms within the crystallization drops (as in this instance) visualization is more improbable. The scale bar corresponds to 50 µm. |