Figure 3
Pgp3 optimization. (a) A large, single Pgp3 crystal grown under metastable conditions using a cognate MIP. Single Pgp3 crystals were obtained using Pgp3-MIPs at dilutions of the original screen `hit' between 50 and 70%. Corresponding controls remained clear. The scale bar corresponds to 75 µm. (b) Clusters of multiple Pgp3 crystals were reproducibly obtained using Pgp3-MIPs when the dilution of the original screen `hit' was greater than 70%. The scale bar corresponds to 100 µm. |