 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 6]](mn5082fig6mag.jpg)
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Figure 6
In vitro interaction of TgENO1 with the TgMAG1 promoter sequence. (a) Oligonucleotide probes were designed for the TTTTCT motif in the bradyzoite TgMAG1 promoter, the TATA box in the human c-Myc promoter and a negative random sequence. (b) SDS–PAGE of purified recombinant TgENO1. Lane 1 contains molecular-weight marker (labelled in kDa). (c) Electrophoretic mobility shift assay of biotinylated TgENO1 incubated with probes and detected for retardation mobility of the DNA–protein complex. The TgENO1–TgMAG1 complex (second lane) was competed with an unlabelled probe prior to incubation with labelled TGMAG1 probe (third lane). |
![[Figure 6]](mn5082fig6.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
