Figure 7
In vitro interaction of TgENO1 and TgENO2 with the bradyzoite TgMAG1 promoter as shown by chromatin immunoprecipitation (ChIP) analyses. (a) Upstream and downstream primers of the TTTTCT motif in TgMAG1 for PCR of the ChIP products. (b) Polyclonal antibodies to bradyzoite-specific TgENO1 precipitated ChIP fragments from the TgMAG1 promoter. (c) Polyclonal antibodies to tachyzoite-specific TgENO2 also pulled down ChIP fragments from TgMAG1 promoter. |