Figure 1
The LLT1 expression construct used for crystallization and its sequence alignment (Clustal Omega; Thompson et al., 1994; Sievers et al., 2011) with mouse Clr-g. The H176C mutation is denoted by an asterisk, glycosylated Asn residues are denoted by triangles and the three pairs of disulfide bonds in LLT1 are numbered. Uncleaved residues of the N-terminal signal sequence and the C-terminal tag are in orange boxes. Secondary structure was assigned by DSSP (Kabsch & Sander, 1983). |