Figure 6
Comparison of published coordinates (blue) and crystal structure models built using Brickworx (red). (a) The GCGA tetraloop from the group II intron IC subdomain (Toor et al., 2008). The model was fitted into the experimentally phased map (3.1 Å resolution) shown contoured at 3.0σ. The model is composed of a model stem generated with 3DNA (Lu & Olson, 2008) and a tetraloop extracted from SSU (PDB entry 1n33
). (b) The sarcin–ricin motif from the lysine riboswitch (Garst et al., 2008) fitted into an experimentally phased map (2.8 Å resolution) shown contoured at 1.8σ. The matched motif was extracted from the LSU structure (PDB entry 3j62
) and further refined in real space with secondary-structure restraints defined using ClaRNA (Waleń et al., 2014). |