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![[Figure 7]](dw5132fig7mag.jpg)
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Figure 7
Dimerization of Tah1 prevents AIP binding. SDS–PAGE gels showing that Tah1 prevents the formation of a Tah1–cHsp90–AIP mixed complex. (a) Control pulldowns in the presence of AIP172–315 (left panel) or full-length AIP, Flag-Tah1 or cHsp90 alone (right panel). (b) Pulldowns with Flag-Tah1 in the presence of cHsp90 and increasing amounts of AIP172–315 (left panel) or full-length AIP (right panel). A fivefold to tenfold excess of either AIP172–315 or full-length AIP does not pull down with the Flag-Tah1–cHsp90 complex, indicating that a three-way complex with AIP does not form. Arrows indicate the positions of the various proteins used. |
![[Figure 7]](dw5132fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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