Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams

Coquelle, N.; Brewster, A.S.; Kapp, U.; Shilova, A.; Weinhausen, B.; Burghammer, M.; Colletier, J.-P.

doi:10.1107/S1399004715004514

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

IUCr IT WDC

home archive editors for authors for readers submit subscribe open access

view article

Figure 1
Sample presentation to the X-ray beam. (a) A brass pin and a washer are glued onto a piece of glass. (b) The concave surface of the first Si₃N₄ wafer is glued onto the washer. (c) 500 nl of crystalline slurry is gently deposited onto the membrane. (d) The second Si₃N₄ wafer is sandwiched over the first. (e) The sandwich is sealed with Araldite resin in order to avoid drying of the material. Following this step, the brass pin is introduced into a magnetic crystal mount. (f) Three-dimensional rendering of steps (a)–(e). (g, h) Overview (g) and close-up view (h) of the lysozyme crystals in the Si₃N₄ sandwich.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 71| Part 5| May 2015| Pages 1184-1196

https://doi.org/10.1107/S1399004715004514

Open

access

Follow Acta Cryst. D

Search IUCr Journals		doi		Advanced search
Author		volume	page