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Figure 5
Specific radiation damage is observed in both the micro and the nano data sets, but does not compromise the structural information. (a) Structure-factor amplitude Fourier difference (Fo − Fo) maps were calculated between our data sets (conservative micro and conservative nano) and four other HEWL data sets: the SFX data set, the Flow-SSX data set and two low-dose data sets collected at either 100 or 295 K. The maps are displayed around the four disulfide bridges of lysozyme, with red and green contours indicating negative and positive density, respectively. (b) Unbiased 2mFo − DFc (blue; contour level 1.0 σ) and mFo − DFc (red and green; contour level ± 3σ) maps are displayed around the four disulfide bridges of lysozyme.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 71| Part 5| May 2015| Pages 1184-1196
https://doi.org/10.1107/S1399004715004514
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