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![[Figure 7]](lp5004fig7mag.jpg)
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Figure 7
Lysophosphatidic acid C18:1 activated endogenous Ca2+-activated chloride channels in Xenopus oocytes when bound to wild-type ginseng major latex-like protein 151 but not when bound to a mutant version in which His147 was replaced by alanine. (a) Representative traces showing the effects of lysophosphatidic acid C18:1 complexed with wild-type ginseng major latex-like protein 151 [GLP(WT)–LPA C18:1] or mutant versions of GLP in which His29, His30, His147 or His148 were replaced with alanine (H29A–LPA C18:1, H30A–LPA C18:1, H147A–LPA C18:1 and H148A–LPA C18:1, respectively), on the activation of Ca2+-activated chloride channels (CaCC). Current traces were recorded at a −80 mV holding potential at the indicated time. (b) Bars represent the peak inward CaCC currents on each complex. Data are shown as means ± SEM (n = 5 for each complex; P < 0.01 and P < 0.001 compared with the wild-type GLP-LPA C18:1 complex are marked * and **, respectively). |
![[Figure 7]](lp5004fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
