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![[Figure 3]](mn5083fig3mag.jpg)
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Figure 3
Long-term stability and pH-dependent affinity of SaBADH (BetB) for NADH at 298 K. (a) SPR signal showing the pH dependence of NADH binding to a fresh SaBADH surface. No changes in RU were detected using two-day-old immobilized SaBADH at any of the pH values except pH 8.0. The inset illustrates the loss of affinity over 12 h using freshly immobilized SaBADH at pH 8.0 and 298 K. (b) pH effect on the interaction of SaBADH with ADP assayed on a two-day-old SaBADH surface at 298 K. (c) Relative response and single binding isotherm fitting of NADH (2 mM) binding to SaBADH in 10 mM HBS, KBS, PBS and TBS buffers at pH 7.0 and 298 K. |
![[Figure 3]](mn5083fig3.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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