Figure 6
Binding of monovalent/divalent cations. (a) Localization of the metal-binding sites within SaBADH (yellow ribbon). Positions of K+ were validated by anomalous difference Fourier maps (insets; red mesh; contoured at 5σ). NAD+ is shown in space-filling representation with atoms colored as follows: C, yellow; O, red; N, blue; P, orange. (b)–(g) Coordination geometries of the metal-binding sites within apo SaBADHBME(+) (b), NAD–SaBADHBME(+) (c, d) and NAD–SaBADHBME(−) (e, f, g). Water molecules are shown as red spheres. |