 | Acta Crystallographica Section D Acta Crystallographica Section D BIOLOGICAL CRYSTALLOGRAPHY |
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![[Figure 1]](mn5091fig1mag.jpg)
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Figure 1
Crystal structure of porcine elastase-cleaved E. coli α2M (ECAM). (a) Schematic representation of the 13 domains of ECAM showing the macroglobulin domains (MG) including the C-terminal MG (CTMG) domain, the bait-region domain (BRD), the complement protein subcomponent (CUB) domain and the thioester domain (TED) containing the CLEQ motif. (b) The structure of elastase-cleaved ECAM with the individual domains coloured as in (a). Smaller van der Waals surfaces in both views are also presented. In the left view the α-helical TED is orientated with the CLEQ thioester (drawn as red van der Waals spheres) positioned above the pocket which is thought to accommodate the attacking protease. (c) Structural alignment of methylamine-treated human α2M (PDB entry 4acq monomer trimmed to the domains present in cleaved ECAM) and elastase-cleaved ECAM (PDB entry 4rtd ) in green and red, respectively. (d) Structural alignment of native SaA2M (PDB entry 4u48 trimmed to the domains present in cleaved ECAM) and elastase-cleaved ECAM (PDB entry 4rtd ) in blue and red, respectively. Structural alignments were performed using the MG domain containing the bait region (shown in orange). |
![[Figure 1]](mn5091fig1.jpg)
| BIOLOGICAL CRYSTALLOGRAPHY |
ISSN: 1399-0047
