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Figure 8
Crystallization of PGAM5 using PEG smears. (a) After MMW and BMW smears were identified as effective precipitants, a deconvoluted screen with single PEGs was performed which identified two PEGs, PEG 3350 and PEG 5K MME, as the effective component in the smears that promoted crystal growth. The specific smear obtained by mixing these two PEGs shows a greater crystallization efficacy towards crystal growth of the wild-type (WT) protein, the wild type–ligand complex and the mutant compared with the two single PEGs, as demonstrated by changes in crystal morphology (b) and the quality and resolution of the collected X-ray diffraction data (c).

Journal logoSTRUCTURAL
ISSN: 2059-7983
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