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Figure 1
TLS refinement suggests macromolecular motions linked to function. (a) Top and side view of GroEL. Each color denotes a unique chain. (b) TLS refinement of GroEL subunits reveals a `tilting' motion around the center of the subunit. (c) GpdQ diffraction image showing significant diffuse scattering features. (d) Refinement of GpdQ fails to produce substantial changes in Rwork and Rfree values between alternate TLS groups. TLS refinement significantly improves the overall Rfree (23.1% pre-TLS).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 71| Part 8| August 2015| Pages 1657-1667
https://doi.org/10.1107/S1399004715007415
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