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Figure 3
Images of 100 nm thick three-dimensional protein crystals produced by a Titan Krios 300 kV FEG transmission EM and captured with a Falcon 2 camera (4096 × 4096 pixels) using an 0.5 s exposure time and an illumination of 3–10 e Å−2. Left, original unprocessed images; right, processed images. (a, b) Lysozyme crystal; (c, d) lysozyme crystal with several ice crystals included (shown in detail); (e, f) crystal of a cross-β peptide. Notice that in the cutout of the results after filtering of the middle image (d) we can distinguish an ice crystal (with a small unit cell and dominant spacings at ±3.8 Å at the left side of the cutout) from the protein crystal (right side of the cutout).

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
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