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Figure 1
Experimental setup for IMISXcryo data collection at 100 K and the steps involved in sample preparation. (a) A view of an IMISX well on a goniometer with the crystal-laden mesophase bolus positioned in the X-­ray beam and in the cryostream at 100 K for SX data collection on beamline PXI (X06SA) at the SLS. The yellow arrow indicates the direction of the X-ray beam. (b) Goniometer base with composite support on the pin (left) ready for attachment of the IMISX well (bottom right). The insert (dashed square) is a view from above of the goniometer base (left) to show the slight curvature in the attached composite support. (c) IMISX well mounted on a goniometer base and seated on a moist paper towel. M, mesophase bolus with crystals; P, precipitant solution; S, composite support. (d) As in (c) where the well has been trimmed to fit into a cryo-vial and to provide access to the precipitant solution. (e) A cotton bud is used to wick away most of the precipitant solution from around the mesophase bolus. A small amount of precipitant is left in place to prevent callapse of the well. The mounted sample is snap-cooled in liquid nitrogen and placed in a cryo-vial for transfer to a Dewar. (f) A view of the well (W) and support (S) in (e) from above to show the slight curvature in the well that confers structural stability to the sample in the cryostream.

Journal logoSTRUCTURAL
ISSN: 2059-7983
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