High-density grids for efficient data collection from multiple crystals

Baxter, E.L.; Aguila, L.; Alonso-Mori, R.; Barnes, C.O.; Bonagura, C.A.; Brehmer, W.; Brunger, A.T.; Calero, G.; Caradoc-Davies, T.T.; Chatterjee, R.; Degrado, W.F.; Fraser, J.S.; Ibrahim, M.; Kern, J.; Kobilka, B.K.; Kruse, A.C.; Larsson, K.M.; Lemke, H.T.; Lyubimov, A.Y.; Manglik, A.; McPhillips, S.E.; Norgren, E.; Pang, S.S.; Soltis, S.M.; Song, J.; Thomaston, J.; Tsai, Y.; Weis, W.I.; Woldeyes, R.A.; Yachandra, V.; Yano, J.; Zouni, A.; Cohen, A.E.

doi:10.1107/S2059798315020847

Acta Crystallographica Section D
Acta Crystallographica
Section D STRUCTURAL BIOLOGY

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Figure 5
Room-temperature experiments with photosystem II crystals in grids. (a) A suspension of photosystem II crystals in a grid port. Polycarbonate backing was applied to one face of the grid, creating a well for a crystal suspension to sit in. A suspension of photosystem II crystals was then pipetted over the open grid ports. (b) Schematic of a cross-section of a grid port prepared for a room-temperature experiment. After filling grid ports with crystals, a second sheet of polycarbonate backing may be applied to the grid to seal the grid ports and protect crystals from dehydration. (c) Diffraction image of a PSII crystal recorded at LCLS-XPP using the grid mount at room temperature. Resolution is indicated by rings, and individual diffraction spots are highlighted with the corresponding resolution.

STRUCTURAL
BIOLOGY

ISSN: 2059-7983

Volume 72| Part 1| January 2016| Pages 2-11

https://doi.org/10.1107/S2059798315020847

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