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accessData-collection strategy for challenging native SAD phasing
aSwiss Light Source, Paul Scherrer Institut, Villigen PSI, Switzerland, bDepartment of Biochemistry, University of Zurich, Zurich, Switzerland, cMembrane Structural and Functional Group, Schools of Medicine and Biochemistry and Immunology, Trinity College, Dublin, Ireland, and dLaboratory of Biomolecular Research, Department of Biology and Chemistry, Paul Scherrer Institut, Villigen PSI, Switzerland
*Correspondence e-mail: vincent.olieric@psi.ch
Recent improvements in data-collection strategies have pushed the limits of native SAD (single-wavelength anomalous diffraction) phasing, a method that uses the weak anomalous signal of light elements naturally present in macromolecules. These involve the merging of multiple data sets from either multiple crystals or from a single crystal collected in multiple orientations at a low X-ray dose. Both approaches yield data of high multiplicity while minimizing radiation damage and systematic error, thus ensuring accurate measurements of the anomalous differences. Here, the combined use of these two strategies is described to solve cases of native SAD phasing that were particular challenges: the integral membrane diacylglycerol kinase (DgkA) with a low Bijvoet ratio of 1% and the large 200 kDa complex of the CRISPR-associated endonuclease (Cas9) bound to guide RNA and target DNA crystallized in the low-symmetry C2. The optimal native SAD data-collection strategy based on systematic measurements performed on the 266 kDa multiprotein/multiligand tubulin complex is discussed.
Keywords: native SAD phasing; anomalous signal; data-collection strategy; complex; membrane protein.
1. Introduction
Native SAD (single-wavelength anomalous diffraction) is a de novo macromolecular structure-determination method in which the is solved by exploiting the anomalous diffraction signal of light atoms present in the crystal (for a review of anomalous diffraction, see Hendrickson, 2014![[Hendrickson, W. A. (2014). Q. Rev. Biophys. 47, 49-93.]](../../../../../../logos/arrows/d_arr.gif "Hendrickson, W. A. (2014). Q. Rev. Biophys. 47, 49-93.")
). Unlike other de novo methods, structure solution by native SAD does not require the incorporation of exogenous heavy atoms, which can be tedious and can lead to non-isomorphism. However, native SAD phasing has its own challenges. Firstly, the where is maximized, is below 2.5 keV (>5 Å) for elements such as sulfur and phosphorus, which is beyond the reach of most current macromolecular crystallography (MX) synchrotron beamlines. Data collection is also hindered by sample and air absorption of X-rays at such low energies. Thus, native SAD data collection is usually performed at `compromise' energies of around 6 keV (2.066 Å; Mueller-Dieckmann et al., 2005; Liu et al., 2014; Weinert et al., 2015) to maximize the anomalous signal while minimizing absorption. Alternatively, home sources, which typically operate with Cu Kα (1.54 Å) or Cr Kα (2.29 Å) radiation, can also be used for such measurements. Secondly, because data collection is performed far from the absorption edges of these light elements, the resulting anomalous signal is small and accurate measurements of the reflection intensities and their differences are essential. To obtain this level of accuracy, random and systematic errors must be minimized. However, typical data-collection protocols at third-generation synchrotrons, usually performed around a single axis, yield anomalous data that can be adversely affected by radiation damage if insufficient care is given to the X-ray dose delivered to the crystal. Consequently, successful cases of phasing by native SAD remain rare; currently, about 150 native SAD structures have been deposited in the Protein Data Bank (Berman et al., 2000).
Recent advances in data-collection strategies have breathed new life into native SAD phasing (reviewed by Rose et al., 2015). The multi-crystal averaging approach involves merging data sets from statistically equivalent crystals (Liu et al., 2012, 2013), enhancing the anomalous signal-to-noise ratio while minimizing anomalous signal decay owing to radiation damage. The key to successful multi-crystal averaging having enough available for data collection. This method is possible because data-merging and data-processing algorithms are robust enough to accommodate a large number of distinct crystal data sets. In particularly challenging cases, data sets from as many as 18 (Akey et al., 2014) and 32 (El Omari et al., 2014) separate crystals were required to obtain sufficient anomalous signal for successful phasing. An extreme case is the recent native SAD phasing of lysozyme using serial crystallography data collected at room temperature, where 2° of data obtained individually from 992 randomly oriented crystals with an average size of 20 µm were merged (Huang et al., 2015). A second approach involves collecting many low-dose data sets (<0.5 MGy per 360°) from a single crystal in multiple orientations, mitigating radiation damage while also reducing systematic errors in data collection (Debreczeni et al., 2003; Brockhauser et al., 2013; Weinert et al., 2015; Finke et al., 2016). By varying the crystal orientation, it is possible to measure the same reflections in different diffraction geometries on different areas of the detector and with different sample absorption, thereby reducing systematic error. This approach has proven to be successful for 11 real-life cases, including a 266 kDa multiprotein/multiligand complex, the largest structure solved by native SAD to date (Weinert et al., 2015). The method benefited from instrumentation developments such as a high-precision PRIGo multi-axis goniometer (Waltersperger et al., 2015) and a photon-counting, noise-free pixel-array detector calibrated for low energies (Henrich et al., 2009), both of which were used in the current study.
Despite the many recent improvements in data-collection strategies, difficult cases such as large substructures, low-symmetry space groups, low diffraction resolution, small crystals, low Bijvoet ratios or some combination thereof still remain a challenge for native SAD phasing. Fortunately, it is possible to combine the two strategies introduced above to extract as much anomalous signal as possible with the highest possible accuracy from as many crystals as needed for successful phasing (Klinke et al., 2015). Here, we present two challenging native SAD cases solved by collecting low-dose data at multiple orientations from three crystals each: the integral membrane diacylglycerol kinase DgkA (Li et al., 2013; 2 × 42 kDa P212121, 2.6 Å resolution) and the large Cas9–RNA–DNA complex (Anders et al., 2014; 200 kDa C2, 2.2 Å resolution). In addition, we demonstrate the benefits of these combined low-dose multi-orientation, multi-crystal data-collection strategies using systematic measurements performed on the multiprotein/multiligand tubulin–RB3–tubulin tyrosine ligase complex T2R-TTL (Prota, Bargsten et al., 2013; Weinert et al., 2015; 266 kDa P212121, 2.3 Å resolution).
2. Materials and methods
2.1. Crystallographic data collection, data processing and analysis
All experiments were performed on the super-bending-magnet beamline X06DA (PXIII) at the Swiss Light Source (SLS), Villigen PSI, Switzerland operating at 2.4 GeV with 400 mA top-up mode. The beamline has a double channel-cut Si(111) monochromator with an energy resolution of 1.4 × 10−4 and an elliptical beam of 90 × 50 µm (FWHM). Data were collected at 100 K with a wavelength of 2.066 Å (6 keV) and a of ∼1.5 × 1010 photons s−1. Multi-orientation data collection was carried out at various χ and φ settings of the multi-axis PRIGo goniometer (Waltersperger et al., 2015) and on a PILATUS 2M-F detector (Henrich et al., 2009) operated in shutterless mode at a frame rate of 10 or 20 Hz and at a sample-to-detector distance of 120 mm. The data were processed using XDS and scaled and merged with XSCALE (Kabsch, 2010). The high-resolution data cutoff was based on the statistical indicators CC1/2 and CC* (Karplus & Diederichs, 2012). determination and phasing were performed with SHELXC/D/E (Sheldrick, 2010) using the HKL2MAP interface (Pape & Schneider, 2004). The anomalous peak heights were calculated using AnoDe without a resolution cutoff (Thorn & Sheldrick, 2011). The number of correct sites found with SHELXD was assessed using SITCOM (Dall'Antonia & Schneider, 2006). and model map cross-correlation calculations were performed using PHENIX (Adams et al., 2010). X-ray dose was estimated with RADDOSE-3D (Zeldin et al., 2013). The Bijvoet ratio was estimated using the formula
![[(|\Delta F^\pm|)/(|F|) = (2N_{\rm A}/N_{\rm P})^{1/2} \times (f''/Z_{\rm eff}), \eqno (1)]](teximages/ba5243fd1.gif)
where f′′ is the imaginary scattering contribution of sulfur (0.95 e at 6 keV), NA is the number of atoms, NP is the number of non-H atoms in the and Zeff is the effective number of electrons of the average protein atom (6.7; Hendrickson & Teeter, 1981). Structure figures were prepared and rendered using PyMOL (DeLano, 2002).
2.1.1. DgkA
P212121 crystals of a DgkA mutant (A41C, C46A, I53V, I70L, M96L, V107D, C113A) measuring ∼100–200 × 50 × 20 µm (Supplementary Fig. S1a) were grown by the in meso or lipid cubic phase method as described elsewhere (Li et al., 2013), harvested and snap-cooled in liquid nitrogen. Three crystals were used for data collection (Supplementary Fig. S1a). From the first crystal, which diffracted to 2.6 Å resolution, a total of ten 360° ω scans were collected with no change in orientation. The second crystal diffracted to 2.8 Å resolution and a total of eight 360° ω scans were collected, including two 360° ω scans with the crystal aligned along the a* axis in order to collect Bijvoet pairs simultaneously on the same image. Data for the third crystal, which diffracted to 2.8 Å resolution, were collected in multiple orientations: four 360° ω scans at χ = 0° and two 360° ω scans each at χ = 10, 20 and 30° (Table 1). At a data-collection speed of 1 deg s−1, the average dose per 360° ω scan was estimated to be 0.5 MGy. Coordinates and structure factors of DgkA have been deposited in the Protein Data Bank (PDB) under accession code 5dwk .
‡For crystals that were translated during measurement, the different positions are labelled p1 and p2. §Friedel pairs are counted as merged reflections. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
2.1.2. Cas9–RNA–DNA
Crystals of a Cas9–sgRNA–target DNA complex in which the complementary (target) DNA strand was mismatched to the sgRNA guide at positions 1–3 were generated, harvested and cryoprotected as described previously (Anders et al., 2014). Three C2 crystals measuring ∼200 × 100 × 50 µm were used for data collection (Supplementary Fig. S1b). The first, second and third crystals diffracted to 2.2, 2.4 and 2.2 Å resolution, respectively, with anomalous signal extending to ∼2.9 Å resolution. The first crystal was large enough to collect two series of multi-orientation data sets from two well separated locations on the crystal (Supplementary Fig. S1b, crystal 1). At each location, a total of eight 360° ω scans were collected with varying χ/φ settings. For the second crystal, a total of eight 360° ω scans were collected at 5° χ increments from 0 to 35° while keeping the φ orientation constant. For the third crystal, four 360° ω scans were collected at φ = 0° and χ = 0, 10, 20 and 30°, and four 360° ω scans were collected at φ = 180° and χ = 0, 10, 20 and 30° (Table 1). At a data-collection speed of 2 deg s−1, the average dose per 360° ω scan was estimated to be 0.25 MGy. The coordinates and structure factors of Cas9–RNA–DNA have been deposited in the PDB under accession code 5fq5 .
2.1.3. T2R-TTL
Rod-shaped P212121 T2R-TTL crystals measuring ∼700 × 100 × 100 µm (Supplementary Fig. S1c) were grown, cryoprotected and snap-cooled in liquid nitrogen as described elsewhere (Prota, Bargsten et al., 2013). Reproducible mounting and cryoprotection was critical as T2R-TTL crystals are extremely sensitive to variation of these conditions (Weinert et al., 2015; PDB entry 4wbn ). Of the 20 crystals screened, only three yielded data suitable for merging based on statistical indicators such at Rmeas and 〈I/σ(I)〉 (Karplus & Diederichs, 2015). The three crystals diffracted to 2.3 Å resolution. From each crystal, data were collected at two different positions that were well separated. At the first position, a single high-dose 360° ω scan, estimated at ∼4 MGy, was collected (Table 1 and Supplementary Data; T2R-TTL high dose, low multiplicity, single orientation). At the second position, eight low-dose 720° ω scans, corresponding to the same accumulated dose of ∼4 MGy (0.25 MGy per 360° ω scan), were collected: seven at different χ increments from 0 to 30° at constant φ and an additional ω scan at χ = 10 or 15° and φ = 90° (Table 1 and Supplementary Data; T2R-TTL low dose, high multiplicity, multi-orientation).
2.2. measurements
(XRF) analysis was performed on the PHOENIX beamline at the Swiss Light Source at an incident radiation energy of 4.1 keV, which is above the Ca K edge (4.038 keV) and not currently accessible at most MX beamlines. The crystal was attached directly to carbon tape and loaded into a 10−4 Pa vacuum chamber. The energy-dispersive spectrum was recorded at room temperature using a single-element solid-state detector (from Roentec) with 180 eV energy resolution. The X-ray beam measuring 100 × 100 µm was directed at the center of the crystal. There was no indication of beam damage over the 120 s collection time. The XRF spectrum was fitted using PyMca (Solé et al., 2007) to extract the elemental composition of the probed crystal volume. The software provides the elemental composition in terms of single peaks corresponding to different atomic energy-level transitions using Gaussian shape emission lines and energy-dependent photoelectric cross-sections.
3. Results and discussion
3.1. Native SAD phasing
3.1.1. DgkA
The integral membrane diacylglycerol kinase DgkA has two trimers in the representing 6 × 130 residues with a total molecular weight of 84 kDa. The has 6 × 2 methionines, 6 × 1 cysteines and a zinc ion (Fig. 1a), corresponding to a theoretical Bijvoet ratio of 1.1% at 6 keV. Previous SAD and MAD attempts at the Zn were unsuccessful. The structure was originally solved by Se-SAD phasing at the Se peak using crystals diffracting to 2.05 Å resolution (Li et al., 2013). The crystals used in the current study only diffracted to 2.6 Å resolution (see §2.1.1). Data were collected both with and without multiple crystal orientations (Table 1). identification using SHELXD was successful with data cut at 4 Å resolution and with E values above 1.5; 14 sites out of 19 were identified, 11 of them correctly as determined using SITCOM with reference PDB entry 3ze3 (Li et al., 2013). The single correct solution obtained after 1000 SHELXD attempts had CCall and CCweak values of 26.2 and 12.0, respectively (Fig. 2a). The correct hand was identified by density modification in SHELXE. Auto-tracing with a search for α-helices resulted in 364 residues (out of 780) built after ten cycles (Fig. 2b), showing a good cross-correlation to the data of 43.95%. The resulting electron-density map (Fig. 3a) showed a model–map cross-correlation of the SHELXE map to the final model of 66.8%.
![[Figure 1]](ba5243fig1thm.gif) | Figure 1 Native SAD structures. (a) DgkA and (b) Cas9–RNA–DNA. The proteins are shown as grey cartoons and the nucleic acid backbones are coloured orange. Anomalous scatterers are depicted as coloured spheres. |
![[Figure 2]](ba5243fig2thm.gif) | Figure 2 SHELX output. (a) SHELXD substructure-determination output for DgkA for 1000 trials. (b) SHELXE output for DgkA after ten cycles of auto-tracing. (c) SHELXD substructure-determination output for Cas9 for 1000 trials. (d) SHELXE output for Cas9 after three cycles of auto-tracing. (e) SHELXD determination for T2R-TTL with 1000 trials. (f) Three cycles of chain tracing with phasing and density modification in SHELXE for T2R-TTL. |
![[Figure 3]](ba5243fig3thm.gif) | Figure 3 Experimental maps after SHELXE phasing and density modification, contoured at 2σ. (a) DgkA map. (b) Cas9–RNA–DNA map. |
Analysis of the data exemplifies the weakness of the anomalous data, mainly owing to the disordered Met66 and Cys41 in chains E and F. The experimental Bijvoet ratio of 1% for this sample was therefore lower than the expected theoretical value of 1.1%.
3.1.2. Cas9–RNA–DNA
The CRISPR-associated protein Cas9 is an RNA-guided endonuclease that has been repurposed for genome editing and gene-expression control. The Cas9–RNA–DNA complex, which crystallizes in the low-symmetry C2, was initially solved with SAD phases obtained from selenomethionine and iridium derivatives (Anders et al., 2014). For native SAD phasing experiments, we used crystals of the Cas9–sgRNA–target DNA complex in which the complementary DNA strand contains mismatches to the guide RNA at positions 1–3 (see §2.1.2). The contains 1371 amino acids, 83 RNA and 39 DNA (total molecular weight ∼200 kDa), with 24 S atoms and 120 P atoms (Fig. 1b), which comprises the largest solved with native SAD phasing to date. In this case, data were collected from three native crystals diffracting to ∼2.2 Å resolution in multiple orientations at a wavelength of 2.066 Å and the data were merged in an initial attempt at locating sulfur and phosphorus positions from the anomalous peaks (Table 1). The solution could be obtained with 1000 SHELXD trials using a high-resolution cutoff of 2.6 Å in a search for 65 sites, excluding data with E values below 1.2 (Fig. 2c). The program initially identified 45 correct sites out of a total of 150 as determined using SITCOM with reference PDB entry 4un5 (Anders et al., 2014). SHELXE completed the to 114 sites, resulting in a very clear hand separation (Fig. 2d). Three cycles of chain tracing with a search for α-helices resulted in a readily interpretable map (Fig. 3b) building a Cα chain of 1060 residues (out of 1372) after three cycles (Fig. 2b) with a cross-correlation of 28.75% to the data. The model–map cross-correlation of the SHELXE map to the final model was 68.0%.
Substructure determination was only successful using data merged from three crystals collected in multiple orientations. The increase in the anomalous peak height resulting from the merging of new data sets (coming from either new orientations of the same crystal or from additional crystals) is shown in Fig. 4. We limited the accumulated dose on each crystal (or crystal location) to a conservative ∼2 MGy in order to minimize the effects of radiation damage. Additional dose brought little additional information; in the case of crystal 1, we observed that the gain in anomalous signal was small beyond 2 MGy and plateaued at 4 MGy (Supplementary Fig. S2).
![[Figure 4]](ba5243fig4thm.gif) | Figure 4 Averaged anomalous peak height of the first 70 anomalous peaks for Cas9–RNA–DNA merged data sets. A total of 3 × 360° data sets were merged from three different crystals. Data from the first crystal were collected at two well separated positions (Supplementary Fig. S1b, crystal 1). The X-ray dose (upper x axis) accumulated at each position was around 2 MGy. |
3.2. analysis
Assigning certain atoms, especially light monoatomic ions, in a can be difficult. An added benefit of data collection at low energy is that the from some of these elements can aid in their correct assignment (Mueller-Dieckmann et al., 2007). In the case of the Cas9–sgRNA–DNA complex, certain ions originally modelled as Mg2+ (PDB entry 4un5 ) displayed strong anomalous signal (>10σ), suggesting alternative atom assignments. However, it was not possible to unambiguously identify the corresponding element from the anomalous peak heights or from an examination of the chemical architecture of the site. One of the crystals was subsequently used to collect a fluorescence spectrum at an incident energy of 4.1 keV (see §2.2). The spectrum confirmed the presence of K+ (Fig. 5), which was likely to arise from the crystallization condition (the buffer solution contained 250 mM KCl and 300 mM KSCN). We therefore reassigned six Mg2+ ions as K+ ions and found an additional one. Subsequent of the contribution f′′, as implemented in phenix.refine (Echols et al., 2014), gave f′′(K+) values between 1.58 and 1.72 e, in agreement with the theoretical value of 1.77 e.
![[Figure 5]](ba5243fig5thm.gif) | Figure 5 Log scale of the X-ray fluorescence spectrum of a Cas9–RNA–DNA crystal recorded at an incident energy of 4.1 keV. The measured spectrum is plotted as black dots and the fit as black (total) and coloured (for different elements) lines. The main contributions (orange) originate from the potassium Kα1,2 (K–LIII and K–LII) and Kβ1 (K–MIII) characteristic lines. The smaller (orange) peaks between 1.5 and 2 keV represent the K–Si escape peaks and are an artifact of the measurement (Papp & Campbell, 2001 ). The Si peak (green line) originates from the crystal holder. |
3.3. Optimal data-collection strategy for challenging native SAD phasing: T2R-TTL as a test case
T2R-TTL is a multiprotein/multiligand complex composed of tubulin (T), the microtubule destabilizing- and stathmin-like protein RB3 and the tubulin-modifying enzyme tubulin tyrosine ligase (TTL; Prota, Magiera et al., 2013). The composed of two α-tubulin, two β-tubulin, one RB3, one TTL, two GTP, two GDP and one AMPPNP molecules, consists of 2317 amino acids (total molecular weight of ∼266 kDa) and has a with 118 S, 13 P, three Ca and two Cl sites. Following our previous successful structure solution of T2R-TTL by native SAD phasing using a single crystal, multi-orientation data-collection strategy (Weinert et al., 2015), we decided to use this system to evaluate the benefits of the combined multi-orientation, multi-crystal strategy. T2R-TTL is an excellent example of a challenging test case; it has a large and a large number of scatterers and, as shown below, it forms crystals of variable quality.
Using multiple native SAD data sets measured from a single T2R-TTL crystal (see §2.1.3 and Supplementary Data), it was not possible to determine the This is likely because the quality of the crystals used in the current work was less than that observed in the original study (Weinert et al., 2015). A solution with SHELXD could only be obtained upon merging data sets from three different crystals each measured in eight different orientations (Table 1 and Supplementary Data) in a search for 75 sites using a resolution cutoff of 3.3 Å and with E values above 1.5 (Fig. 2e). The successful SHELXD substructure solution had a CCall and a CCweak of 36.9 and 18.2, respectively. The number of correct sites, as determined using SITCOM with reference PDB entry 4wbn , was 86 and this increased to 111 using in SHELXE. Density modification resulted in a clear separation of hands (Fig. 2f). Three cycles of chain tracing with a search for α-helices resulted in a readily interpretable map, building a Cα chain of 1669 residues (out of 2319) after three cycles with a cross-correlation of 28.75% to the data. The model–map cross-correlation of the SHELXE map to the final model was 78.5%.
The merit of combining low-dose data from multiple crystals in multiple orientations, which was readily observed in the merging statistics (see Supplementary Data), was assessed by comparing the average anomalous peak heights for four data-collection strategies: (i) high dose, single orientation from a single crystal; (ii) low dose, multiple orientation from a single crystal; (iii) merging high-dose, single-orientation data from three crystals; and (iv) merging low-dose, multiple-orientation data from three crystals (Fig. 6). A single-orientation scan at high dose gave only 23 anomalous peaks above 10σ, which was insufficient for successful solution. Despite a threefold higher accumulated dose, merging high-dose data from three different crystals resulted in 50 anomalous sites above 10σ, similar to merging low-dose, multiple-orientation data from a single crystal. However, this was still insufficient for successful solution. The search was only successful when low-dose, multiple-orientation data sets from three crystals were merged, yielding 70 anomalous sites above 10σ. This is consistent with our previous study, in which solution and hence native SAD phasing were successful by merging low-dose, multiple-orientation scans from a single crystal, giving 80 anomalous sites above 10σ (Weinert et al., 2015). This is also consistent with a study showing that searches tend to be successful when mean peak heights in the anomalous difference map are above a certain threshold (Bunkóczi et al., 2015); for T2R-TTL crystals, this threshold is around 10σ (dashed line in Fig. 6).
![[Figure 6]](ba5243fig6thm.gif) | Figure 6 Comparison of anomalous peak heights for various data-collection strategies. The anomalous peak heights for the first 100 peaks are shown for four data-collection strategies for T2R-TTL data sets: (i) high dose, low multiplicity, single orientation at a single crystal location (red), (ii) low dose, high multiplicity, multiple orientation at a single crystal location (brick), (iii) merging high-dose, single-orientation data from three crystals (blue) and (iv) merging low-dose, multiple-orientation data from three crystals (green). For each crystal, data were collected with the same dose at two well separated positions. The dotted line marks the 10σ threshold. The arrows point to the last peak above 10σ and show the number of sites above this threshold. |
Taken together, there is a clear benefit in combining the two data-collection strategies: (i) a low-dose, multiple-orientation approach efficiently extracts as much anomalous data as possible while minimizing radiation damage and (ii) if the anomalous signal from a single crystal sample is not sufficient for successful phasing, data sets from multiple statistically equivalent crystals can be merged. Combining the two approaches means that fewer crystal samples are required. In addition, for the same accumulated dose, there is no difference in diffraction resolution between data sets collected using a high-dose, single-orientation strategy and merged data sets collected using a low-dose, multiple-orientation strategy (Table 1 and Supplementary Data).
4. Conclusion and outlook
Native SAD could be considered the `ideal' de novo phasing method for macromolecules because it relies solely on information from atoms that are naturally present in the sample. The method is readily applicable to challenging cases. Accordingly, there is every reason to try native SAD first with macromolecules that contain a sufficient number of naturally occurring anomalous scatterers. Here, we show that multi-crystal averaging of data sets used in conjunction with a low-dose, multi-orientation strategy is beneficial in difficult cases where data from one crystal are insufficient. Further, low-dose data collection in multiple orientations means that fewer crystal specimens are needed to provide the required data accuracy.
That said, this combined approach is still in its infancy and there is room for improvement in order to make native SAD more broadly applicable and more generally accepted, especially in cases of low resolution, which are currently dominated by heavy-atom phasing methods. In addition, collecting multiple 360° scans on multiple crystals, as detailed here, takes more time than single-orientation high-dose scans and may be unappealing in situations where synchrotron beamtime is limited. Nevertheless, advances in data-acquisition hardware, in particular detector instrumentation, will rapidly improve the situation. Furthermore, the method described here lends itself to automation.
As the frontiers of crystallography continue to expand, fundamental techniques for solving the will continue to evolve. In particular, new methods such as serial crystallography have presented new challenges that require the development of tools for obtaining the most accurate measurements. Data collection at energies below 6 keV should boost the anomalous signal, but presents technical challenges, especially air absorption and scattering, sample absorption and detector geometry. These are currently being investigated at operational beamlines such as BL-1A at the Photon Factory (see Rose et al., 2015), as well as upcoming beamlines such as I23 at Diamond Light Source (Allan et al., 2015) and LAX at NSLS-II (Hendrickson, 2014).
Footnotes
‡These authors contributed equally to this work.
§Present address: National Center for Protein Sciences, State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People's Republic of China.
Acknowledgements
This work was supported in part by funding from Science Foundation Ireland (12/IA/1255 to MC), by a grant from the Swiss National Science Foundation (310030B_138659 to MOS) and a European Research Council Starting Grant (ANTIVIRNA, Grant no. 337284 to MJ).
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