issue contents

Journal logoSTRUCTURAL
ISSN: 2059-7983

June 2017 issue

Proceedings of the CCP-EM Spring Symposium

Edited by Tom Burnley, Paula da Fonseca and Randy Read

Highlighted illustration

Cover illustration: Cryo-EM has undergone a major `resolution-revolution'. It has helped advance our understanding of a key biological macromolecule, the ribosome. Ribosomes have shared a progressive journey with cryo-EM; in the development of the method and use of the method to understand ribosome structural biology (Javed et al., p. 509). The cover shows a bacterial ribosome map (with independently painted subunits) to highlight the near-atomic details that can be resolved using the current technology thus driving biology forward.


link to html
An introduction to the CCP-EM proceedings special issue of Acta Cryst D.

research papers

link to html
An overview is given of the new CCP-EM software suite, including the underpinning framework, the current functionality and the distribution procedure. This first version of the suite has a focus on building and refining atomic models.

link to html
A technical description of the Dynamo software package for subtomogram averaging is provided. Details are given on advanced MATLAB libraries, parallelization strategies, the use of GPUs and accessibility though the Amazon cloud computing services.

link to html
This paper provides an introduction to the Electron Bio-Imaging Centre (eBIC) at Diamond Light Source: an external user facility established in the UK for high-end cryo-electron microscopy. Details are given of the first year of operation along with highlights and future challenges.

link to html
The formal description of a workflow to cryo-EM structure determination in the RELION program allows standardization of procedures and on-the-fly image processing during data acquisition.

link to html
The Electron Microscopy Data Bank (EMDB), the public archive for three-dimensional EM reconstructions, is an invaluable resource for obtaining a birds-eye view of trends affecting the field of cryo-EM. EMDB is growing rapidly, with almost a quarter of the entries having been released over the past year.

link to html
The structural biology of co-translational protein folding on the ribosome is reviewed.

link to html
High-resolution cryo-EM was used to investigate the structures of inhibitor-bound human and P. falciparum 20S proteasomes, revealing the molecular basis for inhibitor specificity that provides a platform for the development of a potential new class of antimalarials. Here, these studies are reviewed and a detailed description of the methods used for structure determination is provided.

link to html
Recent developments in electron microscopy have provided new opportunities in the field of structure-based drug design. This review looks at the challenges that remain and the future prospects for the use of electron microscopy in this area.

link to html
This review article summarizes recent progress in our understanding of chromatin biology based on single-particle cryo-electron microscopy studies.
Follow Acta Cryst. D
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds