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Figure 2
Statistical analysis of crystallization results. (a) Correlation between crystallization results and crystal-packing propensity for β-sheet. For each PhBCCPΔN79 construct, ratio values of four categories, i.e. the ratios of clear drop (top left), amorphous precipitate (top right), non-analyzable crystal (bottom left) and analyzable crystal (bottom right), from the crystallization screening experiment (horizontal axis) are plotted versus the crystal-packing propensity of residue 138 (vertical axis) with overlaid linear regressions. Labelling for each dot denotes the single-letter code of residue 138. The Pearson correlation coefficient (r) is given in each graph. (b) Correlation between crystal-packing propensity and ratios of crystals. The ratio of obtaining crystals from the crystallization screening experiment was calculated as (number of non-analyzable crystals + analyzable crystals) × 100/528. The ratios of crystals (horizontal axis) are plotted versus the crystal-packing propensity of residue 138 (vertical axis) in the same manner as that shown in (a), except that the crystal-packing propensities of four types calculated from different secondary structures are compared: sheet (top left), helix (top right), coil (bottom left) and all (bottom right). |
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