Conformational dynamics of the essential sensor histidine kinase WalK

The different conformations of WalK reveal the intrinsic dynamic properties of a sensor histidine kinase structure as a molecular basis for multifunctionality.

Introducing site-specific cysteines into nanobodies for mercury labelling allows de novo phasing of their crystal structures

Nanobodies are used as crystallization chaperones and here site-specific mercury labelling of nanobodies is shown as a new tool for phasing.

Structure of the conserved Francisella virulence protein FvfA

1.8 Å resolution crystal structures of a Francisella protein required for growth in host cells are reported.

X-ray and EM structures of a natively glycosylated HIV-1 envelope trimer

Heavily glycosylated structures of an HIV-1 envelope trimer derived by X-ray crystallography and cryo-EM are compared.

Towards a compact and precise sample holder for macromolecular crystallography

New compact and precise cryogenic sample holders for macromolecular crystallography are proposed as possible future European standards.

FlexED8: the first member of a fast and flexible sample-changer family for macromolecular crystallography

A sample changer based on a six-axis industrial robot and a new sample-storage dewar with an ice-cleaning feature have been developed to open automated X-ray crystallography beamlines to new sample-holder models, such as miniSPINE and NewPin, while remaining compatible with the widespread SPINE sample-holder standard.

Mismodeled purines: implicit alternates and hidden Hoogsteens

This paper describes a method that employs difference electron density to identify DNA purines modeled in the incorrect syn/anti conformation. Different classes of mistakes that were identified by using this method to survey all PDB models containing DNA are highlighted.