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![[Figure 7]](cb5096fig7mag.jpg)
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Figure 7
Duck lysozymes superposed on the chicken enzyme. (a, b) Cartoons of Pekin DEL-I (orange) and two crystal forms of DEL-III (both light orange) superposed on HEL (PDB entry 1iee, grey). Amino-acid identities different from the chicken enzyme are shown as sticks. The arrow indicates the active-site cleft. (c–h) Ensemble superposed with the HEL–HyHEL5 complex (PDB entry 1yqv, cyan cartoon and surface) and the HEL–HyHEL10 complex (PDB entry 3d9a, slate cartoon and surface). (e) Position 68 is directly adjacent to HyHEL5, whilst positions 93 and 100 are directly adjacent to HyHEL10. (f) Substitution of Lys49 of the HyHEL10 light chain by threonine enhances binding fivefold to DELs which contain N97R. (g) The salt bridge found in the HEL–HyHEL5 complex is not supported by the R68K substitution in DELs. (h) The S100R substitution found in DEL-III disrupts binding to HyHEL10 owing to a steric clash with the HyHEL10 surface (shaded). |
![[Figure 7]](cb5096fig7.jpg)
 | STRUCTURAL BIOLOGY |
ISSN: 2059-7983
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