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Figure 1
Purification and model fit of YiuA. (a) SDS–PAGE gel showing the enrichment of YiuA over the various steps of purification. Molecular-weight standards are shown on the left (labeled in kDa). Lane 1, uninduced BL21 whole cells. Lane 2, BL21 whole cells induced with IPTG. Lane 3, lysate supernatant fraction following French press cell disruption. Lane 4, Ni-affinity chromatography eluate. Lane 5, anion-exchange chromatography eluate. Lane 6, gel-filtration peak fraction. (b) HiLoad 26/600 Superdex 200 pg gel-filtration chromatogram for YiuA purification. Fractions containing the peak from this chromatogram were concentrated, are represented in lane 6 in (a) and were used for crystallography. (c) Model overlay of 2Fo − Fc electron difference density (bright blue mesh) at site 1.

Journal logoSTRUCTURAL
BIOLOGY
ISSN: 2059-7983
Volume 73| Part 11| November 2017| Pages 921-939
https://doi.org/10.1107/S2059798317015236
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